Pleckstrin homology (PH) domains all contain a seven-standard b-sandwich that allows binding to various species of phosphatidylinositol (PtdIns) phosphates.1 Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(4,5)-P2 Binding Protein contains a highly specific PH domain that recognizes and binds PtdIns-(4,5)-P2 (see Figure 1).2 PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.3,4 PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signaling cascade that has been implicated in cancer5 PtdIns-(4,5)-P2 binding protein can be used in in vitro assays for the detection of PtdIns-(4,5)-P2 in PI3-kinase and PTEN phosphatase assays.WARNING This product is not for human or veterinary use.
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